but I just bought a truck with 100k miles on it and would like to do everything I can to tune it up and increase mileage.
As has been said, plugs, wires, cap and rotor. Good ones. AC Delco, Dayco, or some other established brand. Bottom-shelf parts have a nasty habit of lasting just long enough to be out of warranty when they fail.
Air filter.
Flush the cooling system with distilled water and refill using new hoses and thermostat (make sure to go back with DexCool if yours had it originally).
New fuel filter.
Go ahead and get a new belt tensioner and idler pulley. If you don't need them now you will soon and they are cheap. Same advice on brand as above on ignition components. Get good ones unless you like being stranded.
I'd be tempted to replace the water pump while you have the belt and hoses off. Again, inexpensive and you will probably need one soon anyway. Ditto brand advice from above.
Service the tranny. Better yet, do a complete flush as a simple filter service only gets about a third of the total fluid volume.
Service the rear diff. If nothing else a change to full synthetic will keep noise and future wear to a minimum.
As for injectors, run Chevron fuel for a couple of tanks. Their Techron additive is a proven commodity. If you don't have a Chevron handy, the Techron additive can be purchased by the bottle (I think Wally World carries it).
Check the tire pressure. Mfgs recommended pressures are a comprimise between wear, ride, and economy. Upping the pressure a few pounds will cause the ride to stiffen up a bit but will also improve economy, sometimes by a good bit. In my old Vic, running the tires at the recommended 35 psi resulted in 20.0 hwy mpg. Up the pressure to 40 psi and mpg immediately jumped to 21.5. Ride was noticeably rougher but not obnoxiously so.
And last but not least, one of the best and fastest ways to improve fuel economy is to ease up on the loud pedal.
Brad